Evolution of nucleic acid extraction methods and its role in development of complex human diagnostic solutions with special reference to tuberculosis: A review
1 Department of Infectious Diseases, TB Research Section, Wobble Base Bioresearch Private limited, Pune, India.
2 Institute of Science, Department of Biosciences (Biotechnology), Shri Jagdishprasad Jhabarmal Tibrewala University, Jhunjhunu, Rajasthan, India.
Review Article
Magna Scientia Advanced Research and Reviews, 2023, 09(02), 115–146
Article DOI: 10.30574/msarr.2023.9.2.0166
Publication history:
Received on 31 October 2023; revised on 10 December 2023; accepted on 12 December 2023
Abstract:
Nucleic acid extraction from biological sources has evolved with time and is currently one of the most important contributors to the development of modern human molecular diagnostics. Pure and sufficient quantity of DNA and RNA from limited quantity of cells, which may be fresh or archived, holds key to successful detection of source and cause of many diseases. The challenges of cell disruption to release nucleic acids and the wide range of strategies applied to purify them by removing various enzyme inhibitors are discussed. The demographic spread of the disease of tuberculosis, hurdles faced during transportation of the clinical samples for nucleic acid extraction of Mycobacterium tuberculosis pathogen and different detection technologies supported by the extracted pathogen nucleic acid are reviewed.
Keywords:
DNA; Purification; Extraction; Detection; Tuberculosis.
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