Colorimetric Reverse Transcription-Loop-Mediated Isothermal Amplification (RT-LAMP) in rapid detection of SARS-CoV-2
1 Laboratory of Molecular Diagnosis, Federal University of Viçosa, campus Rio Paranaíba. Road MG 230 Km 7, LAE Building, room 237, Zip code 38810-000, Rio Paranaíba – MG, Brazil.
2 Center of Biotechnology, University of Mogi das Cruzes, Av. Dr. Cândido X. de Almeida e Souza, 200 - Centro Cívico, Zip code 08780-911, Mogi das Cruzes, SP, Brazil.
3 Technological Research Center, University of Mogi das Cruzes, Av. Dr. Cândido X. de Almeida e Souza, 200 - Centro Cívico, Zip code 08780-911, Mogi das Cruzes, SP, Brazil.
Short Communication
Magna Scientia Advanced Research and Reviews, 2022, 04(02), 001–004
Article DOI: 10.30574/msarr.2022.4.2.0026
Publication history:
Received on 11 January 2022; revised on 15 February 2022; accepted on 15 February 2022
Abstract:
Diagnosis by RT-LAMP (Reverse Transcription–Loop-Mediated Isothermal Amplification) is relatively simple, fast and requires inexpensive equipment. The result is visualized with the naked eye, and therefore, has the potential to become popular among molecular tests for viral detection. This work aimed to test and validate different RT-LAMP protocols for diagnostics of SARS-CoV-2. To this end, we used 20 samples that had been tested at the Laboratory of Molecular Diagnostics for SARS-CoV-2 by RT-PCR, with results ranging from “detected to undetected”. The colorimetric RT-LAMP results were compatible with those obtained by RT-PCR in 95% of the cases. The results show good sensitivity and fidelity of the test. Its applicability detecting SARS-CoV-2 can be important for massive testing.
Keywords:
Alternative diagnostics; Covid-19; Viral detection; Molecular diagnostics; RT-qPCR
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Copyright © 2022 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0