Assessment of serum levels of 8-hydroxy-2’ deoxyguanosine and f2-isoprostanes in newly diagnosed adult hypertensive with clinical depression in NAUTH, Nnewi, Nigeria
1 Department of Clinical Chemistry, College of Health Sciences, P.M.B 5025, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria.
2 Department of Medicine, College of Health Sciences, P.M.B 5025, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria.
3 Department of Medical Laboratory Science, Ebonyi State University, Ebonyi State, Nigeria.
4 Department of Radiography & Radiological Sciences, College of Health Sciences, P.M.B 5025, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria.
Research Article
Magna Scientia Advanced Research and Reviews, 2025, 13(01), 104-112
Article DOI: 10.30574/msarr.2025.13.1.0015
Publication history:
Received on 03 December 2024; revised on 08 January 2025; accepted on 10 January 2025
Abstract:
Despite the high prevalence of depression and hypertension, the relationship between the two diseases has received little attention. The potential roles of some biomarkes of oxidative stress in disease progression and management is very crucial. Aim: The present study therefore, evaluated the serum levels of 8-hydroxy-2’ deoxyguanosine (8-OHdG) and F-2 isoprostanes in newly diagnosed hypertensive individuals with and without depression at Nnamdi Azikiwe University Teaching Hospital, Nnewi. Method: This was a cross sectional study carried out on randomly selected 121 consented adult male and female participants within the ages of 18-65 years which comprises 30 newly diagnosed hypertensive individuals without depression (Hypertensive), 31 hypertensive individuals with depression selected from the department of internal medicine. 30 non hypertensive with depression (Depressive) and 30 apparently healthy non-hypertensive individuals without depression which served as control selected among the hospital staff. Their bio-data was obtained from their hospital records. 3 ml of venous blood were collected from each of the participants, dispensed into a plain container, centrifuged and serum separated into another container and stored at -20 0C for the assessment of serum 8-OHdG using Enzyme Linked Immunosorbent Assay (ELISA) Method. Result: The result showed that Hypertensive individuals with and without clinical depression (107.08±22.05, 129.16±82.49) had significantly higher serum level of 8-OHdG when compared with control group (53.95±28.25) (P<0.05 respectively). Hypertensive individuals (129.16±82.49) also had significantly higher 8-OHdG level when compared with non-hypertensive with depression (63.31±37.30) (P<0.05). Hypertensive individuals with depression (9.94±7.14) and depressive individuals (8.99±5.84) also had significantly higher F2-Isoprostanes level when compared with control (5.15±1.47) (P<0.05 respectively). There was a moderately strong positive correlation between 8-OHdG level and body mass index in both hypertensive and depressive individuals (r=0.682, P<0.05). Conclusion: The study observed higher 8-OHdG and F2-Isoprostanes in individuals with hypertension with and without clinical depression. In addition, there is evidence that oxidative stress is related to inflammation and endothelial activation in individuals with both conditions leading to disease severity.
Keywords:
Hypertension; Clinical depression; 8-OHdG; F2-Isoprostanes; Oxidative stress
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