Toxicity of iron oxide nanoparticles, silver nanoparticles and their mixture on antioxidant enzymes and free radicals in male rats

Ahmed Ibrahim Younus1, Mokhtar Ibrahim Yousef2, Maher Abdel-NabiKamel3, Rakhad Alrawi4, * and Jubran Mohammed Abdulrahman5

1 Ministry of Health, Iraq.
2 Department of Environmental Studies, Institute of Graduate Studies and Research, Alexandria University, Egypt.
3 Department of Biochemistry, Medical Research Institute, Alexandria University, Egypt.
4 Grand Canyon University and Arizona College. Arizona, USA.
5 Center of Research and Educational Studies, Ministry of Education, Iraq.
 
Research Article
Magna Scientia Advanced Biology and Pharmacy, 2021, 01(02), 009-020
Article DOI: 10.30574/msabp.2021.1.2.0001
Publication history: 
Received on 02 January 2021; revised on 09 January 2021; accepted on 12 January 2021
 
Abstract: 
In the present study Wistar male rats were used. Rats were divided into 4 equal groups, 10 rats each. Group 1 served as control, group 2 was administered orally with Fe2O3NPs (5 mg/kg BW; >50 nm), group 3 was treated intraperitoneally with AgNPs (50 mg/kg BW; >100 nm) and group 4 was administered with the mixture of Fe2O3NPs with AgNPs. Animals were treated with the doses every day for 79 days. Results showed significant (P < 0.05) decrease in the antioxidant enzymes (GPX, GST, CAT and SOD) and reduced glutathione (GSH) and total antioxidant capacity (TAC), while significant (P < 0.05) increase in thiobarbituric acid-reactive substances (TBARS) and nitric oxide (NO) in plasma and testes of rats treated with Fe2O3NPs, AgNPs and their combination compared to control group.
 
Keywords: 
Iron oxide nanoparticles; Silver nanoparticles; Antioxidant enzymes; Free radicals
 
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