Toxicity of cocoa bean shell extract (Theobroma cacao L.) on the mortality of Aedes aegypti L. Mosquito Larvae
Department of Biology Education, Faculty of Teacher and Education, University of Jember, Jember, Indonesia.
Magna Scientia Advanced Biology and Pharmacy, 2025, 15(01), 016-020
Article DOI: 10.30574/msabp.2025.15.1.0037
Publication history:
Received on 26 March 2025; revised on 03 May 2025; accepted on 06 May 2025
Abstract:
The Aedes aegypti L. mosquito is one of the vectors of Dengue Hemorrhagic Fever. Control of mosquito vectors is expected to have an impact on reducing the population of the mosquito vector Aedes aegypti L. so that it is no longer significant as a disease transmitter. One way to control this mosquito vector is by using biolarvicide which comes from the cocoa bean shell. The aim of this research was to determine the toxicity (LC50) of cocoa bean shell extract on the mortality of Aedes aegypti L. mosquito larvae. The research subjects were 640 Aedes aegypti L. larvae divided into eight treatment groups. The concentrations used in this research were 100 ppm, 400 ppm, 700 ppm, 1000 ppm, 1300 ppm, 1600 ppm, positive control used abate and negative control used distilled water. Observations were carried out within 24 hours. Calculation of the Lethal Concentration 50 (LC50) value used probit analysis. The results of the probit analysis showed that the LC50 value was at a concentration of 787.14 ppm. Cocoa bean shell extract (Theobroma cacao L.) is toxic to Aedes aegypti L. larvae at a concentration of 787.14 ppm. Conclusion: Cocoa bean shell extract (Theobroma cacao L.) at a concentration of 787.14 ppm was able to kill 50% of Aedes aegypti L. larvae within 24 hours
Keywords:
Aedes aegypti L; Cocoa Bean; Shell Extract; Mosquito Larvae
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