Screening of some catalytic activities of mature berries of Solanum aethiopicum cultivar "Klongbo"

Fagbohoun Jean Bedel 1, *, Aké-assi Yolande 2, Djina Yves 3, N’guessan Kouakou Alban 1 and Kouamé Lucien Patrice 3

1 Department of Biochemistry-Genetics, University Peleforo Gon Coulibaly, Korhogo, Côte d’Ivoire.
2 Central Laboratory for Food Hygiene and Agribusiness, LANADA, Abidjan, Côte d’Ivoire.
3 Department of Food Science and Technology, University Nangui Abrogoua, Abidjan, Côte d’Ivoire.
 
Research Article
Magna Scientia Advanced Biology and Pharmacy, 2021, 01(02), 035-041
Article DOI: 10.30574/msabp.2021.1.2.0024
Publication history: 
Received on 29 December 2020; revised on 07 January 2021; accepted on 09 January 2021
 
Abstract: 
Aubergines of the Solanum aethiopicum cultivar "Klongbo" are widely cultivated and consumed by the Ivorian population. However, their post-harvest conservation is problematic. In order to prevent their post-harvest degradation, this study initiates the screening of enzymatic activities responsible for hydrolysis and oxidation activities in the berries of Solanum aethiopicum cultivar "Klongbo". Among the enzymatic activities tested, phosphatases, N'Acethylglucosaminidase, dopamineoxydase and pyrocatecholoxydase were the predominant enzymatic proteins. The optimum hydrolysis and oxidation conditions in terms of pH and temperature are listed as follows: phosphatases (pH 8.0; 50°C), N-acetylases (pH 5.6; 45°C), dopamineoxydase (pH 9.6; 10°C) and pyrocatecholoxydase (pH 9.0; 10°C). Cu2+ ions inhibit all tested enzymatic activities and especially Zn2+ ions for polyphenoloxidase activities. EDTA has an inhibitory effect on phosphatase and N'acetylase activities. SDS has an inhibitory effect on dopamineoxydasic, pyrochatecholoxydasic and phosphatasic activities. On the other hand, it has no effect on N'Acethylglucosaminidasic activities.
 
Keywords: 
Solanum aethiopicum; Polyphenoloxidase; Phosphatase; N'Acethylglucosaminidase
 
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